PYCR1 Monoclonal antibody

PYCR1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66510-1-Ig

产品说明书

CloneNo. 1H2G1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

P5CR 1, P5CR, P5C reductase 1, P5C, EC:1.5.1.2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, COLO 320 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PYCR1 Ready-To-Use IHC Kit. PYCR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66510-1-Ig targets PYCR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PYCR1 fusion protein Ag17919 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 pyrroline-5-carboxylate reductase 1
别名 P5CR 1, P5CR, P5C reductase 1, P5C, EC:1.5.1.2
计算分子量 319 aa, 33.8 kDa
观测分子量 33 kDa, 35 kDa
GenBank蛋白编号BC022244
基因名称 PYCR1
Gene ID (NCBI) 5831
RRIDAB_2881873
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP32322
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa.

实验方案

Product Specific Protocols
WB protocol for PYCR1 antibody 66510-1-IgDownload protocol
IHC protocol for PYCR1 antibody 66510-1-IgDownload protocol
IF protocol for PYCR1 antibody 66510-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Med

Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma

Authors - Yimin Sun
humanIHC

Appl Immunohistochem Mol Morphol

PYCR1, BANF1, and STARD8 Expression in Gastric Carcinoma: A Clinicopathologic, Prognostic, and Immunohistochemical Study

Authors - Ola A Harb
Loading...