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验证数据展示

  • WB analysis of A549 using 55380-1-AP (same clone as 55380-1-PBS)

    WB analysis of A549 using 55380-1-AP (same clone as 55380-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 55380-1-AP (same clone as 55380-1-PBS)

    WB analysis of MCF-7 using 55380-1-AP (same clone as 55380-1-PBS)

    WB result of PYGB antibody (55380-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGB transfected MCF-7 cells. This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 55380-1-AP (same clone as 55380-1-PBS)

    IHC staining of mouse heart using 55380-1-AP (same clone as 55380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PYGB antibody included
  • IF Staining of A549 using 55380-1-AP (same clone as 55380-1-PBS)

    IF Staining of A549 using 55380-1-AP (same clone as 55380-1-PBS)

    Immunofluorescent analysis of A549 cells using 55380-1-AP (PYGB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PYGB Polyclonal antibody, PBS Only

PYGB Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 55380-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PYGB Ready-To-Use IHC Kit. PYGB primary antibody included.

产品信息

55380-1-PBS targets PYGB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphorylase, glycogen; brain
别名
计算分子量 97 kDa
观测分子量 97-105 kDa
GenBank蛋白编号NM_002862
基因名称 PYGB
Gene ID (NCBI) 5834
RRIDAB_11182159
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11216
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PYGB belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. This antibody is specific to PYGB. It has no cross reaction to PYGM and PYGL.