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  • Featured Product
  • KD/KO Validated

PYGB Polyclonal antibody, PBS Only

PYGB Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55380-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of A549 using 55380-1-AP (same clone as 55380-1-PBS)

    WB analysis of A549 using 55380-1-AP (same clone as 55380-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 55380-1-AP (same clone as 55380-1-PBS)

    WB analysis of MCF-7 using 55380-1-AP (same clone as 55380-1-PBS)

    WB result of PYGB antibody (55380-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGB transfected MCF-7 cells. This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 55380-1-AP (same clone as 55380-1-PBS)

    IHC staining of mouse heart using 55380-1-AP (same clone as 55380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PYGB antibody included
  • IF Staining of A549 using 55380-1-AP (same clone as 55380-1-PBS)

    IF Staining of A549 using 55380-1-AP (same clone as 55380-1-PBS)

    Immunofluorescent analysis of A549 cells using 55380-1-AP (PYGB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55380-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PYGB Ready-To-Use IHC Kit. PYGB primary antibody included.

产品信息

55380-1-PBS targets PYGB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphorylase, glycogen; brain
Synonyms
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 97-105 kDa
GenBank Accession NumberNM_002862
Gene Symbol PYGB
Gene ID (NCBI) 5834
RRIDAB_11182159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11216
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PYGB belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. This antibody is specific to PYGB. It has no cross reaction to PYGM and PYGL.