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Perforin Recombinant antibody, PBS Only (Detector)

Perforin Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241009C10

Cat no : 83977-1-PBS

Print datasheet

Synonyms

P1, Lymphocyte pore-forming protein, HPLH2, FLH2, Cytolysin

验证数据展示

  • WB analysis using 83977-1-RR (same clone as 83977-1-PBS)

    WB analysis using 83977-1-RR (same clone as 83977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83977-1-RR (Perforin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83977-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 83977-1-RR (same clone as 83977-1-PBS)

    IHC staining of human tonsillitis using 83977-1-RR (same clone as 83977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83977-1-RR (Perforin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83977-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 83977-1-RR (same clone as 83977-1-PBS)

    IHC staining of human tonsillitis using 83977-1-RR (same clone as 83977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83977-1-RR (Perforin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83977-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00872-1

    Cytometric bead array standard curve of MP00872-1

    Cytometric bead array standard curve of MP00872-1, Perforin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83977-2-PBS. Detection antibody: 83977-1-PBS. Standard: Ag6060. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83977-1-PBS

    Affinity and Kinetic Characterization of 83977-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83977-1-PBS against Human Perforin was performed. The affinity constant is 1.33 nM.

产品信息

83977-1-PBS targets Perforin as part of a matched antibody pair:

MP00872-1: 83977-2-PBS capture and 83977-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Perforin fusion protein Ag6060 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name perforin 1 (pore forming protein)
Synonyms P1, Lymphocyte pore-forming protein, HPLH2, FLH2, Cytolysin
Calculated Molecular Weight 61 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC063043
Gene Symbol Perforin
Gene ID (NCBI) 5551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14222
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Perforin (PRF1) is one of the major cytolytic proteins of cytolytic granules. It is known to be a crucial effector molecule in cytolytic T lymphocyte and natural killer cell-mediated cytotoxicity. This protein has structural and functional similarities to complement component C9. Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. Defects in PRF1 are the cause of familial hemophagocytic lymphohistiocytosis type 2 (FHL2), which is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. (PMID: 2417226; 7774276; 2783486; 10583959)