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Perforin Recombinant antibody

Perforin Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241009C10

Cat no : 83977-1-RR

Print datasheet

Synonyms

P1, Lymphocyte pore-forming protein, HPLH2, FLH2, Cytolysin

验证数据展示

  • WB analysis using 83977-1-RR

    WB analysis using 83977-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83977-1-RR (Perforin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 83977-1-RR

    IHC staining of human tonsillitis using 83977-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83977-1-RR (Perforin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 83977-1-RR

    IHC staining of human tonsillitis using 83977-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83977-1-RR (Perforin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCTLL-2 cells, NK-92 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83977-1-RR targets Perforin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Perforin fusion protein Ag6060 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name perforin 1 (pore forming protein)
Synonyms P1, Lymphocyte pore-forming protein, HPLH2, FLH2, Cytolysin
Calculated Molecular Weight 61 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC063043
Gene Symbol Perforin
Gene ID (NCBI) 5551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14222
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Perforin (PRF1) is one of the major cytolytic proteins of cytolytic granules. It is known to be a crucial effector molecule in cytolytic T lymphocyte and natural killer cell-mediated cytotoxicity. This protein has structural and functional similarities to complement component C9. Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. Defects in PRF1 are the cause of familial hemophagocytic lymphohistiocytosis type 2 (FHL2), which is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. (PMID: 2417226; 7774276; 2783486; 10583959)

实验方案

Product Specific Protocols
WB protocol for Perforin antibody 83977-1-RRDownload protocol
IHC protocol for Perforin antibody 83977-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols