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验证数据展示

  • WB analysis using 81232-8-RR (same clone as 81232-8-PBS)

    WB analysis using 81232-8-RR (same clone as 81232-8-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81232-8-RR (Phospho-AKT (Thr308) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81232-8-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 81232-8-RR (same clone as 81232-8-PBS)

    FC experiment of HeLa using 81232-8-RR (same clone as 81232-8-PBS)

    1x10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Phospho-AKT (Thr308) Recombinant antibody (81232-8-RR, Clone:241845F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81232-8-PBS in a different storage buffer formulation.

Phospho-AKT (Thr308) Recombinant antibody, PBS Only

Phospho-AKT (Thr308) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 81232-8-PBS

产品说明书

CloneNo. 241845F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

AKT pan, AKT 1, AKT (pan), AKT, AKT1

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

产品信息

81232-8-PBS targets Phospho-AKT (Thr308) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-akt murine thymoma viral oncogene homolog 1
别名 AKT pan, AKT 1, AKT (pan), AKT, AKT1
计算分子量 56 kDa
观测分子量60 kDa
GenBank蛋白编号BC000479
基因名称 AKT1
Gene ID (NCBI) 207
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP31749
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The serine-threonine kinase Akt, also known as protein kinase B (PKB), contributes to a broad range of cellular functions including cell survival, proliferation, gene expression and migration of cells of most lineages. AKT has a wide range of cellular substrates, and the oncogenicity of AKT arises from activation of both proliferative and anti-apoptotic signaling, thus making this kinase an attractive target for cancer therapy. Activation of mammalian AKT depends on its recruitment to the membrane through binding of phosphatidylinositol-3,4,5-trisphosphate (PIP3) to the PH domain of AKT, and subsequent phosphorylation at two key residues, Thr308 and Ser473, located at the catalytic domain and C-terminal regulatory domain, respectively. (PMID: 34740102, PMID: 29017516)