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Phospho-AKT (Thr308) Recombinant antibody, PBS Only

Phospho-AKT (Thr308) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241845F1

Cat no : 81232-8-PBS

Print datasheet

Synonyms

AKT pan, AKT 1, AKT (pan), AKT, AKT1

验证数据展示

  • WB analysis using 81232-8-RR (same clone as 81232-8-PBS)

    WB analysis using 81232-8-RR (same clone as 81232-8-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81232-8-RR (Phospho-AKT (Thr308) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81232-8-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 81232-8-RR (same clone as 81232-8-PBS)

    FC experiment of HeLa using 81232-8-RR (same clone as 81232-8-PBS)

    1x10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Phospho-AKT (Thr308) Recombinant antibody (81232-8-RR, Clone:241845F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81232-8-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

产品信息

81232-8-PBS targets Phospho-AKT (Thr308) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name v-akt murine thymoma viral oncogene homolog 1
Synonyms AKT pan, AKT 1, AKT (pan), AKT, AKT1
Calculated Molecular Weight 56 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC000479
Gene Symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31749
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The serine-threonine kinase Akt, also known as protein kinase B (PKB), contributes to a broad range of cellular functions including cell survival, proliferation, gene expression and migration of cells of most lineages. AKT has a wide range of cellular substrates, and the oncogenicity of AKT arises from activation of both proliferative and anti-apoptotic signaling, thus making this kinase an attractive target for cancer therapy. Activation of mammalian AKT depends on its recruitment to the membrane through binding of phosphatidylinositol-3,4,5-trisphosphate (PIP3) to the PH domain of AKT, and subsequent phosphorylation at two key residues, Thr308 and Ser473, located at the catalytic domain and C-terminal regulatory domain, respectively. (PMID: 34740102, PMID: 29017516)