Phospho-AKT (Thr308) Recombinant antibody

Phospho-AKT (Thr308) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 81232-8-RR

产品说明书

CloneNo. 241845F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

Akt (Thr308), AKT1 (phospho T308), p-Akt, p-Akt (Thr308), p-Akt-Thr308

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCalyculin A treated HEK-293 cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81232-8-RR targets Phospho-AKT (Thr308) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-akt murine thymoma viral oncogene homolog 1
别名 Akt (Thr308), AKT1 (phospho T308), p-Akt, p-Akt (Thr308), p-Akt-Thr308
计算分子量 56 kDa
观测分子量60 kDa
GenBank蛋白编号BC000479
基因名称 AKT1
Gene ID (NCBI) 207
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP31749
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The serine-threonine kinase Akt, also known as protein kinase B (PKB), contributes to a broad range of cellular functions including cell survival, proliferation, gene expression and migration of cells of most lineages. AKT has a wide range of cellular substrates, and the oncogenicity of AKT arises from activation of both proliferative and anti-apoptotic signaling, thus making this kinase an attractive target for cancer therapy. Activation of mammalian AKT depends on its recruitment to the membrane through binding of phosphatidylinositol-3,4,5-trisphosphate (PIP3) to the PH domain of AKT, and subsequent phosphorylation at two key residues, Thr308 and Ser473, located at the catalytic domain and C-terminal regulatory domain, respectively. (PMID: 34740102, PMID: 29017516)

实验方案

Product Specific Protocols
WB protocol for Phospho-AKT (Thr308) antibody 81232-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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