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Phospho-AKT (Ser473) Recombinant antibody, PBS Only

Phospho-AKT (Ser473) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2M10

Cat no : 80462-1-PBS

Print datasheet

Synonyms

Phospho-AKT1 (Ser473), AKT pan, AKT 1, AKT (pan), AKT

验证数据展示

  • WB analysis using 80462-1-RR (same clone as 80462-1-PBS)

    WB analysis using 80462-1-RR (same clone as 80462-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80462-1-RR (Phospho-AKT (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80462-1-PBS in a different storage buffer formulation.

  • WB analysis using 80462-1-RR (same clone as 80462-1-PBS)

    WB analysis using 80462-1-RR (same clone as 80462-1-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80462-1-RR (Phospho-AKT (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80462-1-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 80462-1-RR (same clone as 80462-1-PBS)

    FC experiment of NIH/3T3 using 80462-1-RR (same clone as 80462-1-PBS)

    1X10^6 NIH/3T3 cells untreated (dashed line) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-AKT1 (Ser473) (80462-1-RR, Clone:2M10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80462-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

产品信息

80462-1-PBS targets Phospho-AKT (Ser473) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name v-akt murine thymoma viral oncogene homolog 1
Synonyms Phospho-AKT1 (Ser473), AKT pan, AKT 1, AKT (pan), AKT
Observed Molecular Weight56-62 kDa
GenBank Accession NumberNM_005163
Gene Symbol AKT1
Gene ID (NCBI) 207
RRIDAB_2918894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31749
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672). 80462-1-RR specifically recognizes AKT1 phosphorylated at Ser473.