Phospho-AKT1 (Ser473) Recombinant antibody
Phospho-AKT1 (Ser473) Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2M10
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293T cells, HEK-293 cells, NIH/3T3 cells, IGF-1 treated HEK-293T cells, Calyculin A treated HEK-293 cells, Calyculin A treated HeLa cells, Calyculin A treated NIH/3T3 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
80462-1-RR targets Phospho-AKT1 (Ser473) in WB, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | WB, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, mouse |
Cited Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | v-akt murine thymoma viral oncogene homolog 1 |
Synonyms | AKT, AKT1, Phospho-AKT (Ser473), Phospho-AKT1 (Ser473), PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha |
Observed Molecular Weight | 56-62 kDa |
GenBank Accession Number | NM_005163 |
Gene Symbol | AKT1 |
Gene ID (NCBI) | 207 |
RRID | AB_2918894 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P31749 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-AKT1 (Ser473) antibody 80462-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
ACS Omega Interpreting the Pharmacological Mechanisms of Sho-saiko-to on Thyroid Carcinoma through Combining Network Pharmacology and Experimental Evaluation. | ||
Biol Pharm Bull Dictamnine ameliorates DNFB-induced atopic dermatitis like skin lesions in mice by inhibiting M1 macrophage polarization and promoting autophagy |