验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Calyculin A treated HeLa cells |
| Positive FC (Intra) detected in | Calyculin A treated NIH/3T3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-80462 targets Phospho-AKT1 (Ser473) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | v-akt murine thymoma viral oncogene homolog 1 |
| Synonyms | AKT pan, AKT 1, AKT (pan), AKT, AKT1 |
| Observed Molecular Weight | 56-62 kDa |
| GenBank Accession Number | NM_005163 |
| Gene Symbol | AKT1 |
| Gene ID (NCBI) | 207 |
| RRID | AB_2919577 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P31749 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672). 80462-1-RR specifically recognizes AKT1 phosphorylated at Ser473.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Phospho-AKT1 (Ser473) antibody CL488-80462 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |