Phospho-BCL2 (Ser70) Recombinant antibody

Phospho-BCL2 (Ser70) Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230061A8

Cat no : 80771-2-RR

Print datasheet

Synonyms

BCL-2, BCL2, 230061A8



经过测试的应用

Positive WB detected inJurkat cells, paclitaxel treated Jurkat cells, Calyculin A treated Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80771-2-RR targets Phospho-BCL2 (Ser70) in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name B-cell CLL/lymphoma 2
Synonyms BCL-2, BCL2, 230061A8
Calculated Molecular Weight 26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10415
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2 belongs to the Bcl-2 family. The anti-apoptotic protein BCL2 can bind to the pro-apoptotic protein BAX or BAK1 to form heterodimers that modulate apoptosis. BCL2 phosphorylation at Ser70 enhances binding to BAX and BAD and is essential for its anti-apoptotic activity. Conversely, BCL2 also regulates autophagy via interaction with BECN1/Beclin 1; moreover, BCL2 phosphorylation including at Ser70 leads to its dissociation from BECN1 and induction of autophagy. These findings suggest that BCL2 regulates the balance between apoptosis and autophagy. (PMID: 29433359)

实验方案

Product Specific Protocols
WB protocol for Phospho-BCL2 (Ser70) antibody 80771-2-RRDownload protocol
IHC protocol for Phospho-BCL2 (Ser70) antibody 80771-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols