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Phospho-Beta Catenin (Ser552) Polyclonal antibody, PBS Only

Phospho-Beta Catenin (Ser552) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28778-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 28778-1-AP (same clone as 28778-1-PBS)

    WB analysis using 28778-1-AP (same clone as 28778-1-PBS)

    Untreated and Forskolin treated cells were subjected to SDS PAGE followed by western blot with 28778-1-AP (Phospho-Beta Catenin (Ser552) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 28778-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 28778-1-AP (same clone as 28778-1-PBS)

    IF Staining of MCF-7 using 28778-1-AP (same clone as 28778-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Beta Catenin (Ser552) antibody (28778-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28778-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

产品信息

28778-1-PBS targets Phospho-Beta Catenin (Ser552) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Synonyms
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight86-100 kDa
GenBank Accession NumberBC058926
Gene Symbol Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35222
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers(PMID: 22617422, PMID: 18334222). Both AMPK and AKT phosphorylates β-catenin at Ser 552. The phosphorylation of β-catenin at Ser552 can stabilize β-catenin, enhance its nuclear accumulation and increase its transcriptional activity(PMID: 2865606, PMID: 20361929).