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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 28778-1-AP (same clone as 28778-1-PBS)

    WB analysis using 28778-1-AP (same clone as 28778-1-PBS)

    Untreated and Forskolin treated cells were subjected to SDS PAGE followed by western blot with 28778-1-AP (Phospho-Beta Catenin (Ser552) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 28778-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 28778-1-AP (same clone as 28778-1-PBS)

    IF Staining of MCF-7 using 28778-1-AP (same clone as 28778-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Beta Catenin (Ser552) antibody (28778-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28778-1-PBS in a different storage buffer formulation.

Phospho-Beta Catenin (Ser552) Polyclonal antibody, PBS Only

Phospho-Beta Catenin (Ser552) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28778-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated

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Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

产品信息

28778-1-PBS targets Phospho-Beta Catenin (Ser552) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 catenin (cadherin-associated protein), beta 1, 88kDa
别名
计算分子量 781 aa, 86 kDa
观测分子量86-100 kDa
GenBank蛋白编号BC058926
基因名称 Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35222
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers(PMID: 22617422, PMID: 18334222). Both AMPK and AKT phosphorylates β-catenin at Ser 552. The phosphorylation of β-catenin at Ser552 can stabilize β-catenin, enhance its nuclear accumulation and increase its transcriptional activity(PMID: 2865606, PMID: 20361929).