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Phospho-Beta Catenin (Ser675) Recombinant antibody, PBS Only

Phospho-Beta Catenin (Ser675) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6D6

Cat no : 80084-1-PBS

Print datasheet

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286

验证数据展示

  • WB analysis using 80084-1-RR (same clone as 80084-1-PBS)

    WB analysis using 80084-1-RR (same clone as 80084-1-PBS)

    Lambda protein phosphatase treated HEK-293 and NIH/3T3 and Non-treated HEK-293 and NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80084-1-RR (Phospho-Beta Catenin (Ser675) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with Beta Catenin antibody 66379-1-Ig as control. This data was developed using the same antibody clone with 80084-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 80084-1-RR (same clone as 80084-1-PBS)

    IF Staining of MCF-7 using 80084-1-RR (same clone as 80084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (80084-1-RR, Clone: 6D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80084-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 80084-1-RR (same clone as 80084-1-PBS)

    IF Staining of MCF-7 using 80084-1-RR (same clone as 80084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (80084-1-RR, Clone: 6D6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80084-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

产品信息

80084-1-PBS targets Phospho-Beta Catenin (Ser675) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Synonyms b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC058926
Gene Symbol Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2918863
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35222
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity.