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Phospho-CHEK2 (Thr68) Recombinant antibody, PBS Only

Phospho-CHEK2 (Thr68) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1L2

Cat no : 81740-1-PBS

Print datasheet

Synonyms

CDS1, CHEK2, CHK2, HuCds1, LFS2, Phospho-CHEK2 (Thr68), PP1425, RAD53

验证数据展示

  • WB analysis of PC-3 using 81740-1-RR (same clone as 81740-1-PBS)

    WB analysis of PC-3 using 81740-1-RR (same clone as 81740-1-PBS)

    Non-treated PC-3 and MMS treated PC-3 cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 81740-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81740-1-RR (same clone as 81740-1-PBS)

    WB analysis of HeLa using 81740-1-RR (same clone as 81740-1-PBS)

    Non-treated HeLa and UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 81740-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CHEK2 Ready-To-Use IHC Kit. CHEK2 primary antibody included.

产品信息

81740-1-PBS targets Phospho-CHEK2 (Thr68) in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CHK2 checkpoint homolog (S. pombe)
Synonyms CDS1, CHEK2, CHK2, HuCds1, LFS2, Phospho-CHEK2 (Thr68), PP1425, RAD53
Calculated Molecular Weight 61 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC004207
Gene Symbol CHEK2
Gene ID (NCBI) 11200
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO96017
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)