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Phospho-CREB1 (Ser133) Recombinant antibody, PBS Only

Phospho-CREB1 (Ser133) Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5H5

Cat no : 81871-1-PBS

Print datasheet

Synonyms

CREB1, CREB-1, CREB 1, CREB, cAMP-responsive element-binding protein 1

验证数据展示

  • WB analysis of HEK-293 using 81871-1-RR (same clone as 81871-1-PBS)

    WB analysis of HEK-293 using 81871-1-RR (same clone as 81871-1-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:20000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Beta-tubulin antibody as loading control. This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 81871-1-RR (same clone as 81871-1-PBS)

    IHC staining of human prostate cancer using 81871-1-RR (same clone as 81871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CREB1 antibody included
  • IHC staining of human prostate cancer using 81871-1-RR (same clone as 81871-1-PBS)

    IHC staining of human prostate cancer using 81871-1-RR (same clone as 81871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CREB1 antibody included
Planning an IHC experiment? We recommend our IHCeasy CREB1 Ready-To-Use IHC Kit. CREB1 primary antibody included.

产品信息

81871-1-PBS targets Phospho-CREB1 (Ser133) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cAMP responsive element binding protein 1
Synonyms CREB1, CREB-1, CREB 1, CREB, cAMP-responsive element-binding protein 1
Calculated Molecular Weight 341 aa, 35 kDa
Observed Molecular Weight 35-46 kDa
GenBank Accession NumberBC010636
Gene Symbol CREB1
Gene ID (NCBI) 1385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16220
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)