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Phospho-CREB1 (Ser133) Recombinant antibody

Phospho-CREB1 (Ser133) Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5H5

Cat no : 81871-1-RR

Print datasheet

Synonyms

CREB1, CREB-1, CREB 1, CREB, cAMP-responsive element-binding protein 1

验证数据展示

  • WB analysis of HEK-293 using 81871-1-RR

    WB analysis of HEK-293 using 81871-1-RR

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:20000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Beta-tubulin antibody as loading control.

  • IHC staining of human prostate cancer using 81871-1-RR

    IHC staining of human prostate cancer using 81871-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CREB1 antibody included
  • IHC staining of human prostate cancer using 81871-1-RR

    IHC staining of human prostate cancer using 81871-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CREB1 antibody included

经过测试的应用

Positive WB detected inCalyculin A treated HEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CREB1 Ready-To-Use IHC Kit. CREB1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81871-1-RR targets Phospho-CREB1 (Ser133) in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cAMP responsive element binding protein 1
Synonyms CREB1, CREB-1, CREB 1, CREB, cAMP-responsive element-binding protein 1
Calculated Molecular Weight 341 aa, 35 kDa
Observed Molecular Weight 35-46 kDa
GenBank Accession NumberBC010636
Gene Symbol CREB1
Gene ID (NCBI) 1385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16220
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)

实验方案

Product Specific Protocols
WB protocol for Phospho-CREB1 (Ser133) antibody 81871-1-RRDownload protocol
IHC protocol for Phospho-CREB1 (Ser133) antibody 81871-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols