PE-conjugated Phospho-CREB1 (Ser133) Recombinant antibody
Phospho-CREB1 (Ser133) Uni-rAbTM Recombinant Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC (Intra)
Conjugate
PE Fluorescent Dye
CloneNo.
5H5
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | Calyculin A treated HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy CREB1 Ready-To-Use IHC Kit. CREB1 primary antibody included.
推荐稀释比
Application | Dilution |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
PE-81871 targets Phospho-CREB1 (Ser133) in FC (Intra) applications and shows reactivity with human samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | cAMP responsive element binding protein 1 |
Synonyms | CREB1, CREB-1, CREB 1, CREB, cAMP-responsive element-binding protein 1 |
Calculated Molecular Weight | 341 aa, 35 kDa |
Observed Molecular Weight | 35-46 kDa |
GenBank Accession Number | BC010636 |
Gene Symbol | CREB1 |
Gene ID (NCBI) | 1385 |
Conjugate | PE Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P16220 |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)