验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | Calyculin A treated HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-81871 targets Phospho-CREB1 (Ser133) in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cAMP responsive element binding protein 1 |
| 别名 | CREB1, CREB-1, CREB 1, CREB, cAMP-responsive element-binding protein 1 |
| 计算分子量 | 341 aa, 35 kDa |
| 观测分子量 | 35-46 kDa |
| GenBank蛋白编号 | BC010636 |
| 基因名称 | CREB1 |
| Gene ID (NCBI) | 1385 |
| RRID | AB_3083817 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P16220 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)