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验证数据展示

  • WB analysis using 85002-1-RR

    WB analysis using 85002-1-RR

    Non-treated mouse heart tissue, phosphatase inhibitor treated mouse heart tissue and λ phosphatase treated mouse heart tissue were subjected to SDS PAGE followed by western blot with 85001-1-RR (Phospho-Cardiac Troponin I (Ser23/24) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-Cardiac Troponin I (Ser23/24) Recombinant antibody

Phospho-Cardiac Troponin I (Ser23/24) Recombinant Antibody for WB, ELISA
Cat No. 85002-1-RR
CloneNo. 241982B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Cardiac Troponin I (Ser23/24), P-Cardiac Troponin I (Ser23/24), P-Cardiac Troponin I, Phospho-Cardiac Troponin I, Phospho-Troponin I (Cardiac)

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, λ phosphatase treated mouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy TNNI3 Ready-To-Use IHC Kit. TNNI3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85002-1-RR targets Phospho-Cardiac Troponin I (Ser23/24) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name troponin I type 3 (cardiac)
Synonyms Cardiac Troponin I (Ser23/24), P-Cardiac Troponin I (Ser23/24), P-Cardiac Troponin I, Phospho-Cardiac Troponin I, Phospho-Troponin I (Cardiac)
Calculated Molecular Weight 210 aa, 24 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberBC096165
Gene Symbol Cardiac Troponin I
Gene ID (NCBI) 7137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP19429
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cardiac Troponin I (cTnI) is produced by cardiac muscle. To date, most investigative effort in phosphorylation mediated regulation of cTnI function has focused on the actions of PKA and PKC. Evidence from studies in a variety of systems, ranging from reconstituted myofilament proteins to cultured myocytes or transgenic animals with heterologous expression of modified cTnI proteins, indicates that PKA-mediated functional effects (eg, decreased myofilament Ca2 sensitivity) are mediated principally through the phosphorylation of Ser22/23 (equivalent to Ser23/24, including the initiating Met, in the rodent sequence) within the unique N-terminal extension of cTnI. (PMID: 15514163)

实验方案

Product Specific Protocols
WB protocol for Phospho-Cardiac Troponin I (Ser23/24) antibody 85002-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols