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验证数据展示

  • WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

    WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

  • WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

    WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

    Non-treated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80340-1-RR (same clone as 80340-1-PBS)

    FC experiment of HeLa using 80340-1-RR (same clone as 80340-1-PBS)

    1X10^6 HeLa cells untreated (dashed lines) and Calyculin A treated (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-Hamartin/TSC1 (Ser511) (80340-1-RR, Clone:3N6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

Phospho-Hamartin/TSC1 (Ser511) Recombinant antibody, PBS Only

Phospho-Hamartin/TSC1 (Ser511) Uni-rAbTM Recombinant Antibody for Indirect ELISA, WB
Cat No. 80340-1-PBS

产品说明书

CloneNo. 3N6

宿主/亚型

Rabbit / IgG

种属反应性

mouse, human

应用

Indirect ELISA, WB

别名

TSC1, TSC 1, TSC, LAM, KIAA0243

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TSC1 Ready-To-Use IHC Kit. TSC1 primary antibody included.

产品信息

80340-1-PBS targets Phospho-Hamartin/TSC1 (Ser511) in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

经测试应用 Indirect ELISA, WB Application Description
经测试反应性 mouse, human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tuberous sclerosis 1
别名 TSC1, TSC 1, TSC, LAM, KIAA0243
计算分子量 130 kDa
观测分子量150 kDa
GenBank蛋白编号NM_000368
基因名称 Hamartin/TSC1
Gene ID (NCBI) 7248
RRIDAB_2918889
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92574
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).