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Phospho-Hamartin/TSC1 (Ser511) Recombinant antibody, PBS Only

Phospho-Hamartin/TSC1 (Ser511) Uni-rAbTM Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

3N6

Cat no : 80340-1-PBS

Print datasheet

Synonyms

TSC1, TSC 1, TSC, LAM, KIAA0243

验证数据展示

  • WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

    WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

  • WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

    WB analysis using 80340-1-RR (same clone as 80340-1-PBS)

    Non-treated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80340-1-RR (same clone as 80340-1-PBS)

    FC experiment of HeLa using 80340-1-RR (same clone as 80340-1-PBS)

    1X10^6 HeLa cells untreated (dashed lines) and Calyculin A treated (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-Hamartin/TSC1 (Ser511) (80340-1-RR, Clone:3N6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TSC1 Ready-To-Use IHC Kit. TSC1 primary antibody included.

产品信息

80340-1-PBS targets Phospho-Hamartin/TSC1 (Ser511) in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity mouse, human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tuberous sclerosis 1
Synonyms TSC1, TSC 1, TSC, LAM, KIAA0243
Calculated Molecular Weight 130 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberNM_000368
Gene Symbol Hamartin/TSC1
Gene ID (NCBI) 7248
RRIDAB_2918889
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92574
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).