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Phospho-Hamartin/TSC1 (Ser511) Recombinant antibody

Phospho-Hamartin/TSC1 (Ser511) Recombinant Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

FC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3N6

Cat No : 80340-1-RR

Print datasheet

Synonyms

Hamartin, KIAA0243, LAM, Phospho tuberous sclerosis 1, Phospho-Hamartin/TSC1 (Ser511), TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein

验证数据展示

  • WB analysis using 80340-1-RR

    WB analysis using 80340-1-RR

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 80340-1-RR

    WB analysis using 80340-1-RR

    Non-treated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of Hela using 80340-1-RR

    FC experiment of Hela using 80340-1-RR

    1X10^6 Hela cells untreated (dashed lines) and Calyculin A treated (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-Hamartin/TSC1 (Ser511) (80340-1-RR, Clone:3N6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

经过测试的应用

Positive WB detected inNIH/3T3 cells, HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated HEK-293 cells, Calyculin A treated NIH/3T3 cells
Positive FC detected inCalyculin A treated Hela cells
Planning an IHC experiment? We recommend our IHCeasy TSC1 Ready-To-Use IHC Kit. TSC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80340-1-RR targets Phospho-Hamartin/TSC1 (Ser511) in WB, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications FC, WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tuberous sclerosis 1
Synonyms Hamartin, KIAA0243, LAM, Phospho tuberous sclerosis 1, Phospho-Hamartin/TSC1 (Ser511), TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein
Calculated Molecular Weight 130 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberNM_000368
Gene Symbol TSC1
Gene ID (NCBI) 7248
RRIDAB_2918889
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92574
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).

实验方案

Product Specific Protocols
WB protocol for Phospho-Hamartin/TSC1 (Ser511) antibody 80340-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols