CoraLite® Plus 488-conjugated Phospho-Hamartin/TSC1 (Ser511) Recombinant antibody
Phospho-Hamartin/TSC1 (Ser511) Uni-rAbTM Recombinant Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3N6
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | Calyculin A treated HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy TSC1 Ready-To-Use IHC Kit. TSC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80340-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-80340 targets Phospho-Hamartin/TSC1 (Ser511) in FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | tuberous sclerosis 1 |
| Synonyms | TSC1, TSC 1, TSC, LAM, KIAA0243 |
| Calculated Molecular Weight | 130 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | NM_000368 |
| Gene Symbol | Hamartin/TSC1 |
| Gene ID (NCBI) | 7248 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92574 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).