Phospho-Histone H2B (Ser14) Recombinant antibody
Phospho-Histone H2B (Ser14) Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240570A8
验证数据展示
经过测试的应用
Positive WB detected in | PMA treated NIH/3T3 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83811-1-RR targets Phospho-Histone H2B (Ser14) in WB, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | histone cluster 2, H2be |
Synonyms | H2B (Ser14), Histone H2B (Ser14), p H2B, p H2B (Ser14), p Histone H2B |
Calculated Molecular Weight | 14 kDa |
Observed Molecular Weight | 14-17 kDa |
GenBank Accession Number | BC005827 |
Gene Symbol | Histone H2B |
Gene ID (NCBI) | 8349 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q16778 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. In addition, MST1 is known to phosphorylate histone H2B in vitro and has recently been shown to directly phosphorylate histone H2B at Ser14 in vivo. (PMID: 17548476)
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-Histone H2B (Ser14) antibody 83811-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |