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Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) Polyclonal antibody, PBS Only

Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 31133-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HEK-293T using 31133-1-AP (same clone as 31133-1-PBS)

    WB analysis of HEK-293T using 31133-1-AP (same clone as 31133-1-PBS)

    Non-treated and IGF-1 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 31133-1-AP (Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 31133-1-PBS in a different storage buffer formulation.

产品信息

31133-1-PBS targets Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name INSR
Synonyms
Observed Molecular Weight95 kDa
GenBank Accession NumberBC117172
Gene Symbol INSR
Gene ID (NCBI) 3643
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06213
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Insulin plays a crucial role in brain functions such as memory improvement and energy metabolism. The INSR shares a high structural homology with the IGF1R (84% similarity in the tyrosine kinase domain, 45-65% in the ligand-binding domain, and more than 50% in the overall amino acid sequence). In addition, ligand-dependent activation of the INSR and IGF1R activates almost identical downstream signaling cascades. This antibody recognizes the phosphorylation of tyrosine 1150 and 1151 of INSR, as well as the phosphorylation of tyrosine 1135 and 1136 of IGF1R. ((PMID:24434591))