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验证数据展示

  • IF Staining of HeLa using 80086-1-RR (same clone as 80086-1-PBS)

    IF Staining of HeLa using 80086-1-RR (same clone as 80086-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed untreated A549 cells, UV (1 hour) and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-JUN (Ser73) antibody (80086-1-RR, Clone: 4A18) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR004). This data was developed using the same antibody clone with 80086-1-PBS in a different storage buffer formulation.

  • WB analysis using 80086-1-RR (same clone as 80086-1-PBS)

    WB analysis using 80086-1-RR (same clone as 80086-1-PBS)

    Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80086-1-RR (Phospho-JUN (Ser73) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 80086-1-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 80086-1-RR (same clone as 80086-1-PBS)

    FC experiment of NIH/3T3 using 80086-1-RR (same clone as 80086-1-PBS)

    1x10^6 NIH/3T3 cells untreated (dashed lines) or treated with UV were intracellularly stained with 0.13 ug Phospho-JUN (Ser73) Recombinant antibody (80086-1-RR, Clone:4A18) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80086-1-PBS in a different storage buffer formulation.

Phospho-JUN (Ser73) Recombinant antibody, PBS Only

Phospho-JUN (Ser73) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 80086-1-PBS

产品说明书

CloneNo. 4A18

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

AP1, AP 1, JUN, c Jun, Activator protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy JUN Ready-To-Use IHC Kit. JUN primary antibody included.

产品信息

80086-1-PBS targets Phospho-JUN (Ser73) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 jun oncogene
别名 AP1, AP 1, JUN, c Jun, Activator protein 1
计算分子量 331 aa, 36 kDa
观测分子量42-45 kDa
GenBank蛋白编号BC068522
基因名称 JUN
Gene ID (NCBI) 3725
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05412
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis (PMID: 22180088). JUN is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. JUN is a basic leucine zipper (bZIP) transcription factor that acts as homo- or heterodimer, binding to DNA and regulating gene transcription (PMID: 9732876). In additon, extracellular signals can induce post-translational modifications of JUN, resulting in altered transcriptional activity and target gene expression (PMID:8464713). More over, it has uncovered multiple layers of a complex regulatory scheme in which JUN is able to crosstalk, amplify and integrate different signals for tissue development and disease. Jun is predominantly nuclear, ubiquitinated Jun colocalizes with lysosomal proteins (PMID: 15469925). This antibody is raised against synthetic phosphopeptide corresponding to residues surrounding Ser73 of human JUN, which can detect the bands around 42-45 kDa.