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验证数据展示

  • WB analysis using 81667-2-RR (same clone as 81667-2-PBS)

    WB analysis using 81667-2-RR (same clone as 81667-2-PBS)

    Non-treated NIH/3T3 cells and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81667-2-RR (Phospho-JUN (Thr91/93) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81667-2-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 81667-2-RR (same clone as 81667-2-PBS)

    FC experiment of NIH/3T3 using 81667-2-RR (same clone as 81667-2-PBS)

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with UV which intracellularly stained with 0.13 ug Phospho-JUN (Thr91/93) Recombinant antibody (81667-2-RR, Clone:241983H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81667-2-PBS in a different storage buffer formulation.

Phospho-JUN (Thr91/93) Recombinant antibody, PBS Only

Phospho-JUN (Thr91/93) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 81667-2-PBS

产品说明书

CloneNo. 241983H8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

AP1, AP 1, JUN, c Jun, Activator protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy JUN Ready-To-Use IHC Kit. JUN primary antibody included.

产品信息

81667-2-PBS targets Phospho-JUN (Thr91/93) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 jun oncogene
别名 AP1, AP 1, JUN, c Jun, Activator protein 1
计算分子量 331 aa, 36 kDa
观测分子量48 kDa
GenBank蛋白编号BC068522
基因名称 JUN
Gene ID (NCBI) 3725
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP05412
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis.The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. (PMID: 22180088, PMID: 17804415)