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Phospho-MAP2K4 (Ser257/Thr261) Polyclonal antibody

Phospho-MAP2K4 (Ser257/Thr261) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29250-1-AP

Print datasheet

Synonyms

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MEK 4, MEK4, MKK4, Phospho-MAP2K4, Phospho-MAP2K4 (Ser257/Thr261), P-MAP2K4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

验证数据展示

  • WB analysis using 29250-1-AP

    WB analysis using 29250-1-AP

    Non-treated PC-3 cells and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29250-1-AP (Phospho-MAP2K4 (Ser257/Thr261) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPDGF treated PC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

29250-1-AP targets Phospho-MAP2K4 (Ser257/Thr261) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase 4
Synonyms JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MEK 4, MEK4, MKK4, Phospho-MAP2K4, Phospho-MAP2K4 (Ser257/Thr261), P-MAP2K4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1
Calculated Molecular Weight 399 aa, 44 kDa
Observed Molecular Weight44-50 kDa
GenBank Accession NumberBC036032
Gene Symbol MAP2K4
Gene ID (NCBI) 6416
RRIDAB_2918258
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP45985
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitogen-activated protein kinase kinase 4 (MAP2K4), also known as MAP kinase kinase 4 (MKK4), is a second-level kinase in the hierarchical mitogen-activated protein kinase (MAPK) pathway and is regulated by Ras. MAP2K4 has been found to be stably mutated in 3-5 % of cancers, such as ovarian, prostate, and liver cancers. MAP2K4 is activated by MEKK1 or ASK1/TAK1/MLK3. It is unique among all known MAP2Ks in its ability to phosphorylate two different MAPKs, p38 and JNK (c-Jun N-terminal kinase), respectively. (PMID: 24487067, PMID: 33341545)

实验方案

Product Specific Protocols
WB protocol for Phospho-MAP2K4 (Ser257/Thr261) antibody 29250-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Phytomedicine

Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity

Authors - Zhong Xian
View Article
humanWB

J Inorg Biochem

Approaches to selective and potent inhibition of glioblastoma by vanadyl complexes: Inducing mitotic catastrophe and methuosis

Authors - Sha Xu
View Article