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Phospho-MKK7 (Ser271/Thr275) Recombinant antibody, PBS Only

Phospho-MKK7 (Ser271/Thr275) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5E9

Cat no : 80357-1-PBS

Print datasheet

Synonyms

JNK activating kinase 2, JNK kinase 2, JNKK 2, Jnkk2, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MAPKK7, MEK 7, MEK7, MKK7, Phospho-MKK7 (Ser271/Thr275), PRKMK7

验证数据展示

  • WB analysis using 80357-1-RR (same clone as 80357-1-PBS)

    WB analysis using 80357-1-RR (same clone as 80357-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80357-1-RR (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80357-1-PBS in a different storage buffer formulation.

  • WB analysis using 80357-1-RR (same clone as 80357-1-PBS)

    WB analysis using 80357-1-RR (same clone as 80357-1-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80357-1-RR (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80357-1-PBS in a different storage buffer formulation.

产品信息

80357-1-PBS targets Phospho-MKK7 (Ser271/Thr275) in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase kinase 7
Synonyms JNK activating kinase 2, JNK kinase 2, JNKK 2, Jnkk2, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MAPKK7, MEK 7, MEK7, MKK7, Phospho-MKK7 (Ser271/Thr275), PRKMK7
Calculated Molecular Weight 47 kDa
Observed Molecular Weight47-52 kDa
GenBank Accession NumberBC038295
Gene Symbol MKK7
Gene ID (NCBI) 5609
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14733
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dual specificity mitogen-activated protein kinase kinase 7 (MKK7), also known as MEK7 or MAP2K7, is a member of mitogen-activated kinase kinase (MAP2K) subfamily, and a key activator of c-Jun N-terminal kinase (JNK) signaling, a pathway that regulates primarily stress and inflammatory responses. MKK7 activity can be increased by either MKK7-autophosphorylation or phosphorylation of the Ser and Thr residues of the S-X-A-K-T motifs in the Kinase domain by upstream MEKK1, MEKK2, or MLK3.(PMID: 32783966, PMID: 31579105)