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Phospho-MLKL (Ser358) Recombinant antibody, PBS Only

Phospho-MLKL (Ser358) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241469D2

Cat no : 82090-2-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HT-29 using 82090-2-RR (same clone as 82090-2-PBS)

    WB analysis of HT-29 using 82090-2-RR (same clone as 82090-2-PBS)

    Non-treated HT-29 cells, TNF alpha and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 82090-2-RR (Phospho-MLKL (Ser358) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82090-2-PBS in a different storage buffer formulation.

产品信息

82090-2-PBS targets Phospho-MLKL (Ser358) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mixed lineage kinase domain-like
Synonyms
Calculated Molecular Weight 54 kDa
Observed Molecular Weight54 kDa
GenBank Accession NumberXM_005255834
Gene Symbol MLKL
Gene ID (NCBI) 197259
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8NB16
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily. One common method for inducing necroptosis is by treating cells grown in culture with a mixture composed of TNF-α, a Smac-mimetic, that degrades cellular inhibitors of apoptosis (cIAPs) and the pancaspase inhibitor Z-VAD-FMK. This mixture is abbreviated as T/S/Z. It drives necrosome complex formation and RIPK3-dependent phosphorylation of Thr-357 and Ser-358 located within the C-terminal kinase-like domain of human MLKL. (PMID: 28878015)