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验证数据展示

  • WB analysis of HT-29 using 82090-2-RR

    WB analysis of HT-29 using 82090-2-RR

    Non-treated HT-29 cells, TNF alpha and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 82090-2-RR (Phospho-MLKL (Ser358) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

Phospho-MLKL (Ser358) Recombinant antibody

Phospho-MLKL (Ser358) Recombinant Antibody for WB, ELISA
Cat No. 82090-2-RR

Print datasheet

CloneNo. 241469D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inTNF alpha and Calyculin A treated HT-29 cells
Planning an IHC experiment? We recommend our IHCeasy MLKL Ready-To-Use IHC Kit. MLKL primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82090-2-RR targets Phospho-MLKL (Ser358) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mixed lineage kinase domain-like
Synonyms
Calculated Molecular Weight 54 kDa
Observed Molecular Weight54 kDa
GenBank Accession NumberXM_005255834
Gene Symbol MLKL
Gene ID (NCBI) 197259
RRIDAB_3670515
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8NB16
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily. One common method for inducing necroptosis is by treating cells grown in culture with a mixture composed of TNF-α, a Smac-mimetic, that degrades cellular inhibitors of apoptosis (cIAPs) and the pancaspase inhibitor Z-VAD-FMK. This mixture is abbreviated as T/S/Z. It drives necrosome complex formation and RIPK3-dependent phosphorylation of Thr-357 and Ser-358 located within the C-terminal kinase-like domain of human MLKL. (PMID: 28878015)

实验方案

Product Specific Protocols
WB protocol for Phospho-MLKL (Ser358) antibody 82090-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols