验证数据展示
产品信息
28953-1-PBS targets Phospho-MST1 (Thr183)/MST2 (Thr180) in WB, Indirect ELISA applications and shows reactivity with human samples.
经测试应用 | WB, Indirect ELISA Application Description |
经测试反应性 | human |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | serine/threonine kinase 4 |
别名 | |
计算分子量 | 56 kDa |
观测分子量 | 52-56 kDa |
GenBank蛋白编号 | BC005231 |
基因名称 | MST1 |
Gene ID (NCBI) | 6789 |
RRID | AB_2918221 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q13043 |
储存缓冲液 | PBS only , pH 7.3 |
储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases. MST1/2 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the MST1/2 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. MST1 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of MST1 at Thr183 (Thr180 in MST2) in the activation loop is a key activation mechanism for MST1/2 because phosphorylation of Thr183/180 causes the cleavage of MST1 by caspases under apoptotic conditions.