Phospho-MST1 (Thr183)/MST2 (Thr180) Recombinant antibody

Phospho-MST1 (Thr183)/MST2 (Thr180) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 80093-1-RR

产品说明书

CloneNo. 1P6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, FC (Intra), ELISA

MST1 (Thr183)/MST2 (Thr180), p MST1 (Thr183)/MST2 (Thr180), Phospho MST1 (Thr183), Phospho MST1 (Thr183)/MST2 (Thr180), Phospho MST2 (Thr180)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, Calyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells, Staurosporine treated Ramos cells, Staurosporine treated Jurkat cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy STK4 Ready-To-Use IHC Kit. STK4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 6 publications below

产品信息

80093-1-RR targets Phospho-MST1 (Thr183)/MST2 (Thr180) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 serine/threonine kinase 4
别名 MST1 (Thr183)/MST2 (Thr180), p MST1 (Thr183)/MST2 (Thr180), Phospho MST1 (Thr183), Phospho MST1 (Thr183)/MST2 (Thr180), Phospho MST2 (Thr180)
计算分子量 56 kDa
观测分子量 59 kDa
GenBank蛋白编号BC005231
基因名称 MST1
Gene ID (NCBI) 6789
RRIDAB_2918864
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13043
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.

实验方案

Product Specific Protocols
WB protocol for Phospho-MST1 (Thr183)/MST2 (Thr180) antibody 80093-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Ecotoxicol Environ Saf

Role of the hippo signaling pathway in the extracellular matrix degradation of chondrocytes induced by fluoride exposure

Authors - Fang-fang Yu
mouseWB

J Hazard Mater

Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice

Authors - Tianxin Zhao
mouseWB

Nat Commun

KK-LC-1 as a therapeutic target to eliminate ALDH+ stem cells in triple negative breast cancer

Authors - Jiawen Bu
humanWB

Exp Eye Res

Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGFβ Ameliorates high myopia-induced ocular hypertension

Authors - Qianwen Bu
mouseWB

Biochim Biophys Acta Mol Basis Dis

Hippo pathway activated by circulating reactive oxygen species mediates cardiac diastolic dysfunction after acute kidney injury

Authors - Xiao Han
ratWB

Cells

Activation of Yes-Associated Protein Is Indispensable for Transformation of Kidney Fibroblasts into Myofibroblasts during Repeated Administration of Cisplatin

Authors - Jia-Bin Yu
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