CoraLite® Plus 647-conjugated Phospho-MST1 (Thr183)/MST2 (Thr180) Recombinant antibody
Phospho-MST1 (Thr183)/MST2 (Thr180) Uni-rAbTM Recombinant Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1P6
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | Calyculin A treated HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy STK4 Ready-To-Use IHC Kit. STK4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80093-1-PBS
推荐稀释比
Application | Dilution |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-80093 targets Phospho-MST1 (Thr183)/MST2 (Thr180) in FC (Intra) applications and shows reactivity with human samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | serine/threonine kinase 4 |
Synonyms | MST1, MST2, STK4, STK4/MST1, MST 2 |
Calculated Molecular Weight | 56 kDa |
Observed Molecular Weight | 59 kDa |
GenBank Accession Number | BC005231 |
Gene Symbol | MST1 |
Gene ID (NCBI) | 6789 |
RRID | AB_2920328 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13043 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors.
Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.