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验证数据展示

  • WB analysis using 82335-1-RR (same clone as 82335-1-PBS)

    WB analysis using 82335-1-RR (same clone as 82335-1-PBS)

    Non-treated and Calyculin A treated various cells were subjected to SDS PAGE followed by western blot with 82335-1-RR (Phospho-NF-κB p65 (Ser468) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin antibody (66031-1-Ig) and NF-κB p65 antibody (80979-1-RR) subsequently. This data was developed using the same antibody clone with 82335-1-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 82335-1-RR (same clone as 82335-1-PBS)

    FC experiment of NIH/3T3 using 82335-1-RR (same clone as 82335-1-PBS)

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-NF-κB p65 (Ser468) Recombinant antibody (82335-1-RR, Clone:6N1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 82335-1-PBS in a different storage buffer formulation.

Phospho-NF-κB p65 (Ser468) Recombinant antibody, PBS Only

Phospho-NF-κB p65 (Ser468) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82335-1-PBS

产品说明书

CloneNo. 6N1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

NF-κB (Ser468), NF-κB p65 (Ser468), p NF-κB, p NF-κB (Ser468), p NF-κB p65

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NF-κB p65/RELA Ready-To-Use IHC Kit. NF-κB p65/RELA primary antibody included.

产品信息

82335-1-PBS targets Phospho-NF-κB p65 (Ser468) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-rel reticuloendotheliosis viral oncogene homolog A (avian)
别名 NF-κB (Ser468), NF-κB p65 (Ser468), p NF-κB, p NF-κB (Ser468), p NF-κB p65
计算分子量 65 kDa
观测分子量75 kDa
GenBank蛋白编号BC011603
基因名称 NF-κB p65
Gene ID (NCBI) 5970
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ04206
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.