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Phospho-NF-κB p65 (Ser536) Recombinant antibody, PBS Only

Phospho-NF-κB p65 (Ser536) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240777D9

Cat no : 80379-2-PBS

Print datasheet

Synonyms

NF κB, NF-κB p65, p65, p65; RELA, RELA

验证数据展示

  • WB analysis of NIH/3T3 using 80379-2-RR (same clone as 80379-2-PBS)

    WB analysis of NIH/3T3 using 80379-2-RR (same clone as 80379-2-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80379-2-RR (Phospho-NF-κB p65 (Ser536) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80379-2-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 80379-2-RR (same clone as 80379-2-PBS)

    FC experiment of PC-3 using 80379-2-RR (same clone as 80379-2-PBS)

    1x10^6 untreated or Calyculin A treated PC-3 cells were intracellularly stained with 0.25 ug Phospho-NF-κB p65 (Ser536) Recombinant antibody (80379-2-RR, Clone:240777D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue), 1x10^6 untreated PC-3 cells were intracellularly stained with 0.25 ug Phospho-NF-κB p65 (Ser536) Recombinant antibody (80379-2-RR, Clone:240777D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(black). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80379-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NF-κB p65/RELA Ready-To-Use IHC Kit. NF-κB p65/RELA primary antibody included.

产品信息

80379-2-PBS targets Phospho-NF-κB p65 (Ser536) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Synonyms NF κB, NF-κB p65, p65, p65; RELA, RELA
Calculated Molecular Weight 65 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC011603
Gene Symbol NF-κB p65
Gene ID (NCBI) 5970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ04206
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.