验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells |
| Positive FC (Intra) detected in | PC-3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
产品信息
80379-2-RR targets Phospho-NF-κB p65 (Ser536) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, FC (Intra), ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | v-rel reticuloendotheliosis viral oncogene homolog A (avian) |
| Synonyms | NF-κB p65 (Ser536), NF-κB p65 Ser-536, Phospho NF-κB p65 (Ser536), phospho-NF-κB p65, Phospho-NF-κB p65 Ser536 |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC011603 |
| Gene Symbol | NF-κB p65 |
| Gene ID (NCBI) | 5970 |
| RRID | AB_3670476 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q04206 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-NF-κB p65 (Ser536) antibody 80379-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Nanomedicine Unexpected Inhibitory Role of Silica Nanoparticles on Lung Cancer Development by Promoting M1 Polarization of Macrophages | ||
Front Immunol SQLE-mediated squalene metabolism promotes tumor immune evasion in pancreatic cancer | ||
Cell Death Discov 17β-estradiol alleviated ferroptotic neuroinflammation by suppressing ATF4 in mouse model of Parkinson's disease | ||
Adv Mater Suppression of Sepsis Cytokine Storm by Escherichia Coli Cell Wall-Derived Carbon Dots | ||
Front Vet Sci Overexpression of the Mas1 gene mitigated LPS-induced inflammatory injury in mammary epithelial cells by inhibiting the NF-κB/MAPKs signaling pathways | ||
Microbiol Spectr Mutation of Pseudomonas aeruginosa lasI/rhlI diminishes its cytotoxicity, oxidative stress, inflammation, and apoptosis on THP-1 macrophages |
