验证数据展示
经过测试的应用
| Positive WB detected in | pervanadate treated HeLa cells |
This antibody recognizes TrkA-II (isoform 1) phosphorylation at Tyr496 or TrkA-I (isoform 2) phosphorylation at Tyr490.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84976-1-RR targets Phospho-TrkA (Tyr496)/TrkB (Tyr516) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | neurotrophic tyrosine kinase, receptor, type 1 |
| 别名 | Phospho-NTRK1, NTRK1 (Tyr496), Phospho-NTRK1 (Tyr496), Phospho-NTRK1-Tyr496, Phospho-TrkA |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC062580 |
| 基因名称 | NTRK1 |
| Gene ID (NCBI) | 4914 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P04629 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The tyrosine kinase receptor TrkA (or neurotrophin receptor kinase 1, NTRK1) has been predominately studied for its role during the development of the nervous system. In developing neurons, TrkA activation upon binding of its ligands nerve growth factor (NGF) or neurotrophin-3 (NT-3) results in the stimulation of various tyrosine kinase-induced signaling pathways leading to neuronal outgrowth. (PMID: 32957504) This antibody recognizes the phosphorylation of TrkA at site 496, which is the phosphorylation of the TrkA-I(isoform 2) sequence at site 490.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TrkA (Tyr496)/TrkB (Tyr516) antibody 84976-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |