验证数据展示
产品信息
67826-1-PBS targets Phospho-P53 (Ser15) as part of a matched antibody pair:
MP50182-1: 67826-1-PBS capture and 60283-2-PBS detection (validated in Cytometric bead array)
MP50183-1: 67826-1-PBS capture and 67900-1-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
经测试应用 | WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description |
经测试反应性 | human |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | tumor protein p53 |
别名 | TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13 |
计算分子量 | 44 kDa |
观测分子量 | 53 kDa |
GenBank蛋白编号 | BC003596 |
基因名称 | P53 |
Gene ID (NCBI) | 7157 |
RRID | AB_2918588 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P04637 |
储存缓冲液 | PBS only , pH 7.3 |
储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)