Phospho-P53 (Ser15) Monoclonal antibody

Phospho-P53 (Ser15) Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (4)

Applications

FC, IF, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H6G1

Cat No : 67826-1-Ig

Print datasheet

Synonyms

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser15), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53



经过测试的应用

Positive WB detected inCalyculin A treated HT-29 cells, HT-29 cells, HEK-293T cells, HEK-293T cells, Etoposide treated HT-29 cells
Positive IF detected inetoposide treated HT-29 cells
Positive FC detected inUV treated HT-29 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67826-1-Ig targets Phospho-P53 (Ser15) in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IF, WB, ELISA
Cited Applications IF, WB
Tested Reactivity Human
Cited Reactivityhuman, mouse, rat, pig
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor protein p53
Synonyms Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser15), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53
Calculated Molecular Weight 44 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC003596
Gene Symbol TP53
Gene ID (NCBI) 7157
RRIDAB_2918588
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

实验方案

Product Specific Protocols
WB protocol for Phospho-P53 (Ser15) antibody 67826-1-IgDownload protocol
IF protocol for Phospho-P53 (Ser15) antibody 67826-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Virol

Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription

Authors - Xiazhen Xu
mouse,pigWB

Food Chem Toxicol

Enhanced glutathione production protects against zearalenone-induced oxidative stress and ferroptosis in female reproductive system

Authors - Wei Fu
humanWB

Front Pharmacol

A New Chalcone Derivative C49 Reverses Doxorubicin Resistance in MCF-7/DOX Cells by Inhibiting P-Glycoprotein Expression.

Authors - Ting Wang
humanWB

Toxicol Lett

The mechanism of rh-endostatin-induced cardiotoxicity and its protection by dihydromyricetin[in vivo/in vitro, C57BL/6 mice, AC16 and hiPSC-CMs]

Authors - Xiaoran Guan
ratWB

World Neurosurg

N-acetylserotonin protects rat nucleus pulposus cells against oxidative stress injury by activating the PI3K/AKT signaling pathway

Authors - Wang Yidian
humanWB

Front Pharmacol

Simultaneous suppression of PKM2 and PHGDH elicits synergistic anti-cancer effect in NSCLC

Authors - Kaixuan Wang