验证数据展示
经过测试的应用
Positive WB detected in | Calyculin A treated HT-29 cells, HT-29 cells, HEK-293 cells, HEK-293t cells, Etoposide treated HT-29 cells, Calyculin A treated HEK-293 cells, UV treated HEK-293T cells |
Positive IF/ICC detected in | etoposide treated HT-29 cells |
Positive FC (Intra) detected in | UV treated HT-29 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67826-1-Ig targets Phospho-P53 (Ser15) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human |
文献引用反应性 | human, mouse, rat, pig |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | tumor protein p53 |
别名 | TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13 |
计算分子量 | 44 kDa |
观测分子量 | 53 kDa |
GenBank蛋白编号 | BC003596 |
基因名称 | P53 |
Gene ID (NCBI) | 7157 |
RRID | AB_2918588 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P04637 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-P53 (Ser15) antibody 67826-1-Ig | Download protocol |
IF protocol for Phospho-P53 (Ser15) antibody 67826-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Phytomedicine MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice | ||
J Virol Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription | ||
Food Chem Toxicol Enhanced glutathione production protects against zearalenone-induced oxidative stress and ferroptosis in female reproductive system | ||
Front Pharmacol A New Chalcone Derivative C49 Reverses Doxorubicin Resistance in MCF-7/DOX Cells by Inhibiting P-Glycoprotein Expression. | ||
Toxicol Lett The mechanism of rh-endostatin-induced cardiotoxicity and its protection by dihydromyricetin[in vivo/in vitro, C57BL/6 mice, AC16 and hiPSC-CMs] | ||
World Neurosurg N-acetylserotonin protects rat nucleus pulposus cells against oxidative stress injury by activating the PI3K/AKT signaling pathway |