Phospho-P53 (Ser15) Monoclonal antibody

Phospho-P53 (Ser15) Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 67826-1-Ig

产品说明书

CloneNo. 1H6G1

宿主/亚型

Mouse / IgG1

种属反应性

human and More (3)

应用

WB, IF/ICC, FC (Intra), ELISA and More (2)

TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
规格: 

-/ -


经过测试的应用

Positive WB detected inCalyculin A treated HT-29 cells, HT-29 cells, HEK-293 cells, HEK-293t cells, Etoposide treated HT-29 cells, Calyculin A treated HEK-293 cells, UV treated HEK-293T cells
Positive IF/ICC detected inetoposide treated HT-29 cells
Positive FC (Intra) detected inUV treated HT-29 cells
Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67826-1-Ig targets Phospho-P53 (Ser15) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse, rat, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tumor protein p53
别名 TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13
计算分子量 44 kDa
观测分子量53 kDa
GenBank蛋白编号BC003596
基因名称 P53
Gene ID (NCBI) 7157
RRIDAB_2918588
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04637
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

实验方案

Product Specific Protocols
WB protocol for Phospho-P53 (Ser15) antibody 67826-1-IgDownload protocol
IF protocol for Phospho-P53 (Ser15) antibody 67826-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Phytomedicine

MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice

Authors - Jia Shi
humanWB

J Virol

Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription

Authors - Xiazhen Xu
mouse,pigWB

Food Chem Toxicol

Enhanced glutathione production protects against zearalenone-induced oxidative stress and ferroptosis in female reproductive system

Authors - Wei Fu
humanWB

Front Pharmacol

A New Chalcone Derivative C49 Reverses Doxorubicin Resistance in MCF-7/DOX Cells by Inhibiting P-Glycoprotein Expression.

Authors - Ting Wang
humanWB

Toxicol Lett

The mechanism of rh-endostatin-induced cardiotoxicity and its protection by dihydromyricetin[in vivo/in vitro, C57BL/6 mice, AC16 and hiPSC-CMs]

Authors - Xiaoran Guan
ratWB

World Neurosurg

N-acetylserotonin protects rat nucleus pulposus cells against oxidative stress injury by activating the PI3K/AKT signaling pathway

Authors - Wang Yidian
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