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CoraLite®555-conjugated Phospho-P53 (Ser15) Monoclonal antibody

Phospho-P53 (Ser15) Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1H6G1

Cat no : CL555-67826

Print datasheet

Synonyms

TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

验证数据展示

  • FC experiment of HT-29 using CL555-67826

    FC experiment of HT-29 using CL555-67826

    1X10^6 UV treated HT-29 cells were intracellularly stained with 0.25 ug CoraLite®555 Anti-Human Phospho-P53 (Ser15) (CL555-67826, Clone:1H6G1) (red), or 0.25 ug CoraLite® Plus 555 Mouse IgG1 Isotype Control (1F8D3) (CL555-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

经过测试的应用

Positive FC (Intra) detected inUV treated HT-29 cells
Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67826-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67826 targets Phospho-P53 (Ser15) in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor protein p53
Synonyms TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13
Calculated Molecular Weight 44 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC003596
Gene Symbol P53
Gene ID (NCBI) 7157
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP04637
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)