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Phospho-P53 (Ser392) Recombinant antibody

Phospho-P53 (Ser392) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241575F6

Cat no : 80077-2-RR

Print datasheet

Synonyms

p P53 Ser392, p P53 (Ser392), p P53, P53 (Ser392), Phospho P53

验证数据展示

  • WB analysis of HEK-293 using 80077-2-RR

    WB analysis of HEK-293 using 80077-2-RR

    Phosphatase inhibitor treated or λ phosphatase treated or untreated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80077-2-RR (Phospho-P53 (Ser392) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 80077-2-RR

    IF Staining of A431 using 80077-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Phospho-P53 (Ser392) antibody (80077-2-RR, Clone: 241575F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 80077-2-RR

    FC experiment of HeLa using 80077-2-RR

    1x10^6 λ phosphatase treated HeLa cells were intracellularly stained with 0.2 ug Phospho-P53 (Ser392) Recombinant Antibody (80077-2-RR, Clone:241575F6)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9)(blue) and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated HeLa cells were intracellularly stained with 0.2 ug Phospho-P53 (Ser392) Recombinant Antibody (80077-2-RR, Clone:241575F6) and PE-conjugated Goat Anti-Rabbit IgG(black). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

经过测试的应用

Positive WB detected inλ phosphatase treated HEK-293 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inλ phosphatase treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80077-2-RR targets Phospho-P53 (Ser392) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor protein p53
Synonyms p P53 Ser392, p P53 (Ser392), p P53, P53 (Ser392), Phospho P53
Calculated Molecular Weight 44 kDa
GenBank Accession NumberBC003596
Gene Symbol P53
Gene ID (NCBI) 7157
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP04637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P53 is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)

实验方案

Product Specific Protocols
WB protocol for Phospho-P53 (Ser392) antibody 80077-2-RRDownload protocol
IF protocol for Phospho-P53 (Ser392) antibody 80077-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols