CoraLite® Plus 488-conjugated Phospho-P53 (Ser46) Monoclonal antibody
Phospho-P53 (Ser46) Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1D10A12
验证数据展示
经过测试的应用
Positive IF/ICC detected in | etoposide treated HT-29 cells |
Positive FC (Intra) detected in | UV treated HT-29 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67900 targets Phospho-P53 (Ser46) in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | tumor protein p53 |
Synonyms | TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13 |
Calculated Molecular Weight | 44 kDa |
Observed Molecular Weight | 53 kDa |
GenBank Accession Number | BC003596 |
Gene Symbol | P53 |
Gene ID (NCBI) | 7157 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P04637 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Phospho-P53 (Ser46) antibody CL488-67900 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |