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Host
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验证数据展示

  • WB analysis using 85044-1-RR (same clone as 85044-1-PBS)

    WB analysis using 85044-1-RR (same clone as 85044-1-PBS)

    Non-treated RAW 264.7 cells and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85044-1-PBS in a different storage buffer formulation.

  • WB analysis using 85044-1-RR (same clone as 85044-1-PBS)

    WB analysis using 85044-1-RR (same clone as 85044-1-PBS)

    Non-treated C6 cells and pervanadate treated C6 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85044-1-PBS in a different storage buffer formulation.

Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant antibody, PBS Only

Phospho-PAK1 (Ser144)/PAK2 (Ser141) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85044-1-PBS

产品说明书

CloneNo. 242123D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

PAK1, p21-activated kinase 1, p21 activated kinase 1, EC:2.7.11.1, Alpha-PAK

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PAK1 Ready-To-Use IHC Kit. PAK1 primary antibody included.

产品信息

85044-1-PBS targets Phospho-PAK1 (Ser144)/PAK2 (Ser141) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 1
别名 PAK1, p21-activated kinase 1, p21 activated kinase 1, EC:2.7.11.1, Alpha-PAK
计算分子量 553 aa, 62 kDa
观测分子量61 kDa
GenBank蛋白编号BC109299
基因名称 PAK1
Gene ID (NCBI) 5058
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13153
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The human PAK family is divided into the group I (PAK1 to PAK3) and group II (PAK4 to PAK6). Group I PAK share some domains that are not present in the group II members. In particular, the autoinhibitory domain (AID) is important for regulation of the kinase activity of the group I family members. Autophosphorylation at PAK1 Ser144, or at the equivalent sites for the other PAK, stabilizes the open conformation and sustains high kinase activity. Mutation of tyrosines 131 or 429 is associated with reduced dimerization and enhanced kinase activity. (PMID: 31748572)