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验证数据展示

  • WB analysis using 85044-1-RR

    WB analysis using 85044-1-RR

    Non-treated RAW 264.7 cells and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • WB analysis using 85044-1-RR

    WB analysis using 85044-1-RR

    Non-treated C6 cells and pervanadate treated C6 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant antibody

Phospho-PAK1 (Ser144)/PAK2 (Ser141) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85044-1-RR

产品说明书

CloneNo. 242123D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

PAK1 (Ser144), PAK1 (Ser144)/PAK2 (Ser141), PAK2 (Ser141), Phospho-PAK1, Phospho-PAK1 (Ser144)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpervanadate treated C6 cells, LPS treated RAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy PAK1 Ready-To-Use IHC Kit. PAK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85044-1-RR targets Phospho-PAK1 (Ser144)/PAK2 (Ser141) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 1
别名 PAK1 (Ser144), PAK1 (Ser144)/PAK2 (Ser141), PAK2 (Ser141), Phospho-PAK1, Phospho-PAK1 (Ser144)
计算分子量 553 aa, 62 kDa
观测分子量61 kDa
GenBank蛋白编号BC109299
基因名称 PAK1
Gene ID (NCBI) 5058
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13153
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The human PAK family is divided into the group I (PAK1 to PAK3) and group II (PAK4 to PAK6). Group I PAK share some domains that are not present in the group II members. In particular, the autoinhibitory domain (AID) is important for regulation of the kinase activity of the group I family members. Autophosphorylation at PAK1 Ser144, or at the equivalent sites for the other PAK, stabilizes the open conformation and sustains high kinase activity. Mutation of tyrosines 131 or 429 is associated with reduced dimerization and enhanced kinase activity. (PMID: 31748572)

实验方案

Product Specific Protocols
WB protocol for Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody 85044-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols