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Phospho-PDH E1 Alpha (Ser293) Polyclonal antibody

Phospho-PDH E1 Alpha (Ser293) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29580-1-AP

Print datasheet

Synonyms

PDHA, PDHA1, PDHCE1A, PDHE1 A type I, PHE1A

验证数据展示

  • WB analysis using 29580-1-AP

    WB analysis using 29580-1-AP

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29580-1-AP (Phospho-PDH E1 Alpha (Ser293) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PDH E1 Alpha (18068-1-AP) antibody as a loading control.

经过测试的应用

Positive WB detected inHeLa cells, λ phosphatase treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PDHA1 Ready-To-Use IHC Kit. PDHA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29580-1-AP targets Phospho-PDH E1 Alpha (Ser293) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Synonyms PDHA, PDHA1, PDHCE1A, PDHE1 A type I, PHE1A
Calculated Molecular Weight 43 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC002406
Gene Symbol PDHA1
Gene ID (NCBI) 5160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08559
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A. Multiple post-translational modifications (PTMs) of PDHA1 have been identified, such as acetylation, succinylation, and tyrosine phosphorylation. However, most of these PTMs ultimately affect PDHA1 activity through their influence on the phosphorylation of Ser293, Ser232, and Ser300 which are regulated by PDKs(pyruvate dehydrogenase kinases) and PDPs(pyruvate dehydrogenase phosphatases).(PMID: 34667275)

实验方案

Product Specific Protocols
WB protocol for Phospho-PDH E1 Alpha (Ser293) antibody 29580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols