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Phospho-PDPK1 (Ser241) Polyclonal antibody, PBS Only

Phospho-PDPK1 (Ser241) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29241-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 29241-1-AP (same clone as 29241-1-PBS)

    WB analysis using 29241-1-AP (same clone as 29241-1-PBS)

    Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29241-1-AP (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:1000 incubated at 4℃ overnight. This data was developed using the same antibody clone with 29241-1-PBS in a different storage buffer formulation.

  • WB analysis using 29241-1-AP (same clone as 29241-1-PBS)

    WB analysis using 29241-1-AP (same clone as 29241-1-PBS)

    Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29241-1-AP (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 29241-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PDPK1 Ready-To-Use IHC Kit. PDPK1 primary antibody included.

产品信息

29241-1-PBS targets Phospho-PDPK1 (Ser241) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 3-phosphoinositide dependent protein kinase-1
Synonyms
Calculated Molecular Weight 556 aa, 63 kDa
Observed Molecular Weight60-63 kDa
GenBank Accession NumberBC012103
Gene Symbol PDPK1
Gene ID (NCBI) 5170
RRIDAB_2918256
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15530
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.