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Phospho-PKC Delta (Thr507) Polyclonal antibody, PBS Only

Phospho-PKC Delta (Thr507) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29564-1-PBS

Print datasheet

Synonyms

PKC delta, PRKCD, PKCD, nPKC-delta, MAY1

验证数据展示

  • WB analysis using 29564-1-AP (same clone as 29564-1-PBS)

    WB analysis using 29564-1-AP (same clone as 29564-1-PBS)

    Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC Delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently. This data was developed using the same antibody clone with 29564-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PRKCD Ready-To-Use IHC Kit. PRKCD primary antibody included.

产品信息

29564-1-PBS targets Phospho-PKC Delta (Thr507) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase C, delta
Synonyms PKC delta, PRKCD, PKCD, nPKC-delta, MAY1
Calculated Molecular Weight 78 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC043350
Gene Symbol PKC Delta
Gene ID (NCBI) 5580
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05655
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PKC Delta(PRKCD) is a member of the PKC family, a phospholipid-dependent family of serine/threonine kinases, and is present in human neutrophils. Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-507 (activation loop of the kinase domain), Ser-645 (turn motif) and Ser-664 (hydrophobic region), need to be phosphorylated for its full activation. This antibody is equivalent to Thr505 in mice.