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验证数据展示

  • WB analysis using 31139-1-AP

    WB analysis using 31139-1-AP

    Phosphatase inhibitor treated HeLa cells, λ phosphatase treated HeLa cells, and non-treated HeLa cells, were subjected to SDS PAGE followed by western blot with 31139-1-AP (Phospho-POLR2A (Ser7) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

Phospho-POLR2A (Ser7) Polyclonal antibody

Phospho-POLR2A (Ser7) Polyclonal Antibody for WB, ELISA
Cat No. 31139-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

hRPB220, hsRPB1, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, λ phosphatase treated HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31139-1-AP targets Phospho-POLR2A (Ser7) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Synonyms hRPB220, hsRPB1, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2
Calculated Molecular Weight 217 kDa
Observed Molecular Weight260 kDa
GenBank Accession NumberNM_000937
Gene Symbol POLR2A
Gene ID (NCBI) 5430
RRIDAB_3669869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24928
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing.

实验方案

Product Specific Protocols
WB protocol for Phospho-POLR2A (Ser7) antibody 31139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols