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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 29874-1-AP (same clone as 29874-1-PBS)

    WB analysis using 29874-1-AP (same clone as 29874-1-PBS)

    Non-treated and Calyculin A treated various cells were subjected to SDS PAGE followed by western blot with 29874-1-AP (Phospho-PPP1CA (Thr320) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control. This data was developed using the same antibody clone with 29874-1-PBS in a different storage buffer formulation.

Phospho-PPP1CA (Thr320) Polyclonal antibody, PBS Only

Phospho-PPP1CA (Thr320) Polyclonal Antibody for WB, Indirect ELISA
Cat No. 29874-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PPP1CA Ready-To-Use IHC Kit. PPP1CA primary antibody included.

产品信息

29874-1-PBS targets Phospho-PPP1CA (Thr320) in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, catalytic subunit, alpha isoform
别名
计算分子量 330 aa, 38 kDa
观测分子量37 kDa
GenBank蛋白编号BC001888
基因名称 PPP1CA
Gene ID (NCBI) 5499
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62136
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166).