Phospho-PTEN (Thr382/383) Polyclonal antibody

Phospho-PTEN (Thr382/383) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29246-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IF/ICC, ELISA

p PTEN, p PTEN (Thr382/383), p PTEN Thr382/383, Phospho PTEN, Phospho PTEN (Thr382/383)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inNIH/3T3 cells, λ phosphatase treated NIH/3T3 cells
Positive IF/ICC detected inλ phosphatase treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PTEN Ready-To-Use IHC Kit. PTEN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 6 publications below

产品信息

29246-1-AP targets Phospho-PTEN (Thr382/383) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatase and tensin homolog
别名 p PTEN, p PTEN (Thr382/383), p PTEN Thr382/383, Phospho PTEN, Phospho PTEN (Thr382/383)
计算分子量 47 kDa
观测分子量55-70 kDa
GenBank蛋白编号BC005821
基因名称 PTEN
Gene ID (NCBI) 5728
RRIDAB_2918257
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60484
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTEN is one of the most critical tumor suppressors, which functions at different subcellular locations, including the plasma membrane and nucleus. The PTEN protein is located at different subcellular regions-PTEN at the plasma membrane suppresses PI3-kinase signaling in cell growth, whereas PTEN in the nucleus maintains genome integrity. At the plasma membrane, PTEN counteracts PI3 kinase signaling by dephosphorylating the potent second messenger PIP3 to PIP2. The loss of PTEN in cancer cells results in over-activation of AKT and mTOR signaling, leading to excessive stimulation of cell growth and inhibition of cell death. In the nucleus, PTEN functions in DNA repair, genome stability, and cell cycle control through associations with Rad51 and p53. PTEN stability is primarily regulated by phosphorylation of C-terminal tail domains (Thr366, Ser370, Ser380, Thr382, Thr383, and Ser385). The phosphorylation leads to a "closed" state of PTEN and maintains PTEN stability. Dephosphorylation of the C-terminal tail opens the PTEN phosphatase domain, thereby increasing PTEN activity. PTEN protein is of the apparent molecular mass expected for PTEN (55 kDa) and PTENα (70 kDa).(PMID: 33083717, PMID: 20622047, PMID: 24768297)

实验方案

Product Specific Protocols
WB protocol for Phospho-PTEN (Thr382/383) antibody 29246-1-APDownload protocol
IF protocol for Phospho-PTEN (Thr382/383) antibody 29246-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Rep

Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells

Authors - Fenghua Qian
humanWB

EMBO Rep

LINC00493-encoded microprotein SMIM26 exerts anti-metastatic activity in renal cell carcinoma

Authors - Kun Meng
pigWB

J Transl Med

The miR-15b-5p/miR-379-3p-FOXO axis regulates cell cycle and apoptosis in scleral remodeling during experimental myopia

Authors - Ruixue Zhang
humanWB

Phytomedicine

Rosmarinic acid attenuates glioblastoma cells and spheroids' growth and EMT/stem-like state by PTEN/PI3K/AKT downregulation and ERK-induced apoptosis

Authors - Aslıhan Şengelen
human,mouseWB

Phytomedicine

Isovitexin alleviates hepatic fibrosis by regulating miR-21-mediated PI3K/Akt signaling and glutathione metabolic pathway: based on transcriptomics and metabolomics

Authors - Yushen Huang
humanWB

J Endocrinol Invest

CircNDST1 promotes papillary thyroid cancer progression via its interaction with CSNK2A1 to activate the PI3K-Akt pathway and epithelial-mesenchymal transition

Authors - C Shu
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