IHCeasy PTEN Ready-To-Use IHC Kit

PTEN Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC0280

Synonyms

10q23del, Bannayan Zonana syndrome, BC005821, BZS, ITGA 2, MGC11227, MHAM, MMAC1, Multiple hamartoma (Cowden syndrome), phosphatase and tensin homolog, PTEN, PTEN1, TEP1



Product Information

KHC0280 is a ready-to-use IHC kit for staining of PTEN. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy PTEN Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

PTEN(Phosphatase and tensin homolog) is also named as MMAC1, TEP1 and it modulates cell cycle progression and cell survival (blockage) through down-regulating the positive cell cycle-regulator (cycle D1) by its phosphatase activity and up-regulating the negative cell cycle regulator p21 (by its lipid phosphatase activity). PTEN is an important tumor suppressor whose inactivation frequency in cancers is ranked second after p53 tumor suppressor inactivation. PTEN is a 403-residue protein structured in an amino (N)-terminal phosphatase domain and a carboxy (C)-terminal C2 domain that binds phospholipid membranes. There are some report showing that SUMOylation appears to be a positive regulator in controlling PTEN membrane association, whereas phosphorylation is a negative regulator that may neutralize SUMOylation through intramolecular electrostatic interactions.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms 10q23del, Bannayan Zonana syndrome, BC005821, BZS, ITGA 2, MGC11227, MHAM, MMAC1, Multiple hamartoma (Cowden syndrome), phosphatase and tensin homolog, PTEN, PTEN1, TEP1