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Phospho-S6 Ribosomal protein (Ser240/244) Polyclonal antibody

Phospho-S6 Ribosomal protein (Ser240/244) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 29220-1-AP

Print datasheet

Synonyms

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, Phospho-RPS6 (Ser240/244), Phospho-S6 Ribosomal Protein (Ser240/244), ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein

验证数据展示

  • WB analysis using 29220-1-AP

    WB analysis using 29220-1-AP

    Serum-free treated NIH/3T3 cells and 10 % fetal bovine serum treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29220-1-AP (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

经过测试的应用

Positive WB detected in10 % fetal bovine serum treated NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29220-1-AP targets Phospho-S6 Ribosomal protein (Ser240/244) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, ELISA
Tested Reactivity Human, Mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S6
Synonyms 40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, Phospho-RPS6 (Ser240/244), Phospho-S6 Ribosomal Protein (Ser240/244), ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein
Observed Molecular Weight32 kDa
GenBank Accession NumberNM_001010
Gene Symbol RPS6
Gene ID (NCBI) 6194
RRIDAB_2918252
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62753
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein S6 (RPS6), also named as Phosphoprotein NP33, may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens and is dephosphorylated at growth arrest.

实验方案

Product Specific Protocols
WB protocol for Phospho-S6 Ribosomal protein (Ser240/244) antibody 29220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols